This is the last straw!
4 seconds sound clip from the Peter Pan (1953) movie soundboard.
You can hear this line at 00:07:42 in the Blu-ray version of the movie.
Quote context
[...]
- Young lady, this is your last night in the nursery.
- And that's my last word on the matter...
- Yes!
- There'll be no more dogs for nursemaids in this house!
[...]