Sir, negative, sir.
3 seconds sound clip from the Jaws (1975) movie soundboard.
You can hear this line at 00:18:39 in the Blu-ray version of the movie.
Quote context
[...]
- Well, Private Joker, I don't believe I heard you correctly.
- Sir, the private said, No, sir, sir.
- Why, you little maggot. You make me wanna vomit.
- Now, you do love the Virgin Mary, don't you?
- Private Joker, are you trying to offend me?
- Who's your squad leader, scumbag?
[...]