What's the matter? Can't you talk? Has the cat got your tongue?
6 seconds sound clip from the Symphony In Slang (Tex Avery, 1951) movie soundboard.
You can hear this line at 00:06:24 in the Blu-ray version of the movie.
Quote context
[...]
- The groom had his hands full too.
- So all this struck me so funny that I died laughin'.
- Well, what do you think? Did you follow me?
- Well, I, uh, I.. Hmm, huh, well, I, uh...
- What's the matter? Can't you talk? Has the cat got your tongue?
- Whoa, whoa! Oh, come on, horse! Whoa!
[...]