No, sir, I won't do it.

4 seconds sound clip from the Ruggles of Red Gap (1935) movie soundboard.

You can hear this line at 01:07:41 in the Blu-ray version of the movie.

Quote context

[...]

- Ye Gods and little prairie dogs!

- Where in... Where did you get that horse blanket?

- Now, look-a-here, this is a man's suit and I aim to wear it!

- Where are your good clothes?

- They're all gone.

- Egbert Floud, take off those clothes.

- No, sir, I won't do it.

- Take off those clothes!

- No, sir, I won't do it! We might just as well have a showdown right here and now.

- What did Lincoln say at Gettysburg? Yeah, you don't know. Well, I'll tell you. He said that all men are created equal. He didn't just mean a few men. He meant all men, and that includes me. I'm created equal.

- Equal to what?

- Equal... equal to what? Well, equal to...

- She changed the subject on me.

[...]