Yes, that's the sign of the werewolf.

2 seconds sound clip from the The Wolf Man (1941) movie soundboard.

You can hear this line at 00:11:19.760 in the Blu-ray version of the movie.

Quote context

[...]

- Tell you what we'll do. We'll take a little walk tonight and we'll talk it over.

- No.

- See you at 8:00.

- No!

- Gypsies, huh?

- Yes, they're fortune-tellers. They pass through here every autumn.

- You know, I haven't had my fortune told in years. How about tonight?

- No.

- Fine, I'll be here at 8:00.

- Yes, that's the sign of the werewolf.

- That's just a legend, though, isn't it?

- Yes, but like most legends, it must have some basis in fact.

- It's probably an ancient explanation of the dual personality in each of us.

- How does it go?

- 'Even a man who is pure in heart and says his prayers by night may become a wolf when the wolfsbane blooms, and the autumn moon is bright'.

[...]