It's Marmaduke.
4 seconds sound clip from the Ruggles of Red Gap (1935) movie soundboard.
You can hear this line at 00:44:30 in the Blu-ray version of the movie.
Quote context
[...]
- Here, here, Bill! That's, a little close even for Red Gap!
- Colonel Ruggles, what did Mr Henshaw say your first name is?
- Oh, that's nice. Mine's Prunella.
- Well, Ruggles, enjoying yourself?
- Well, that's fine. What prompted you to mingle with the guests?
- Oh, sir, I had definite instructions from Mr Floud, sir, to mix.
- I'm sorry to spoil your pleasure, but you must come home with me at once.
[...]