Because only Spartan women give birth to real men.

— Queen Gorgo (Lena Headey)

5 seconds sound clip from the 300 movie soundboard.

You can hear this line at 00:09:42 in the Blu-ray version of the movie.

Quote context

[...]

- My king and queen, I was just entertaining your guests.

- I'm sure.

- Before you speak, Persian, know that in Sparta everyone, even a king's messenger is held accountable for the words of his voice. Now, what message do you bring?

- Earth and water.

- You rode all the way from Persia for earth and water?

- Do not be coy or stupid, Persian. You can afford neither in Sparta.

- What makes this woman think she can speak among men?

- Because only Spartan women give birth to real men.

- Let us walk to cool our tongues.

- If you value your lives over your complete annihilation, listen carefully, Leonidas.

- Xerxes conquers and controls everything he rests his eyes upon.

- He leads an army so massive, it shakes the ground with its march. So vast, it drinks the rivers dry.

[...]