Gentlemen, please, rest your sphincters.
4 seconds sound clip from the Blazing Saddles (1974) movie soundboard.
You can hear this line at 00:20:55 in the Blu-ray version of the movie.
Quote context
[...]
- A hrumph! A hrumph! A hrumph!
- I didn't get a hrumph out of that guy!
- Hrumph!
- Gentlemen, please, rest your sphincters.
- Oh, I am sorry, sir. I didn't mean to overstep my bounds. You say that.
[...]