- As you know, the loss of heat from the brain is the most reliable way of determining time of death. Right, Chief?
- Oh, yeah.

13 seconds sound clip from the In the Heat of the Night (1967) movie soundboard.

You can hear this line at 00:25:08.725 in the Blu-ray version of the movie.

Quote context

[...]

- It's 4:45. What time was this man killed?

- Well, Sam Wood found him on the street about 3:00 a.m. The doctor here says it's possible he was killed an hour earlier.

- At 2:00?

- Maybe a little later... 2:15, 2:30.

- Would you feel the face and jaw, please? Am I mistaken, or has rigor begun?

- It has.

- You'll notice, too, that postmortem lividity is present here in the lower portions. So the time of death really has to be earlier, wouldn't you say?

- Well, we'll be able to pinpoint that as soon as I get a thermometer.

- As you know, the loss of heat from the brain is the most reliable way of determining time of death. Right, Chief?

- Oh, yeah.

- Well... which one of you gentlemen will assist me?

(Phone ringing) It's for you, Chief.

- Yeah, talk to me. When? Oh, good. Yeah, yeah, right away.

- I've got a runaway suspect. Boy, I gotta go.

[...]