Don't you try to scare me, Ant, because I walk where fools fear to tread.

4 seconds sound clip from The Ant and the Aardvark (1969) - The Ant from Uncle (S1E3).

You can hear this line at 00:05:39.406 in the Blu-ray version of the episode.

Quote context

[...]

- The one sure way to hurt an ant is to plug up his anthill.

- You're just wasting your time, daddy-o, because I moved in with my relatives, the Gi-Ants.

- This anthill is sort of a condominium of sorts.

- So now you're trying to make anthills out of mountains.

- You better be careful, Dad.

- Don't you try to scare me, Ant, because I walk where fools fear to tread.

- Okay. Either you leave now or I'll be forced to come in and get you.

- What do you want, shorty?

- Nothing.

- I thank you, Minerva, a lot.

- That's all right, Charlie.

[...]