No!

3 seconds sound clip from the Joe Dirt (2001) movie soundboard.

You can hear this line at 00:47:24 in the Blu-ray version of the movie.

Quote context

[...]

- Wait, wait, man. I found out later that she wasn't my sister.

- Oh, thank God!

- Thank God, dude! That was gonna be a little much.

- So I did a real bad thing there, 'cause I think you're my sister.

- Is that all?

- No!

- My family's last name is Buckwalter. My brother's name is Cletis.

- So, you see, we're not related. We can have sex again.

- Joe, what's the matter? Don't I turn you on?

- I don't know what the problem is.

[...]