Yep. Apparently, back in the day, Charlie had a little sexual encounter with this one's ma. Say hello to Charlie Two.

9 seconds sound clip from the Joe Dirt (2001) movie soundboard.

You can hear this line at 01:24:14 in the Blu-ray version of the movie.

Quote context

[...]

- My Hemi! You got it outta hock for me? Dang! Yeah!

- Somebody else wants to get in here.

- Who's that?

- Come on, boy! Come on!

- Wow! Looks like Charlie!

- Yep. Apparently, back in the day, Charlie had a little sexual encounter with this one's ma. Say hello to Charlie Two.

- Good boy.

- You got a big family now, Joe Dirt. You better be good to us.

- Oh, I will.

- Hey, Dirt! I thought I told you, buddy, nobody wants you around!

[...]