- What won't run long without winding? Clock? Stairway! A river?
- The Gotham river.

14 seconds sound clip from Batman (1966) - A Riddle a Day Keeps the Riddler Away (S1E11) TV Series.

You can hear this line at 00:16:35 in the Blu-ray version of the movie.

Quote context

[...]

- That's no riddle.

- But those are.

- How much dirt is in a hole three acres square and 200 feet deep?

- None. Because it's a hole.

- I suggest that's a bit too elementary for our quizzical criminal.

- If you're having difficulty with the first, perhaps your young friend can help you on this one.

- What won't run long without winding?

- What won't run long without winding? Clock? Stairway! A river?

- The Gotham river.

- We'll soon find out. Come on, Robin. Back to the Batcave!

- Gotham City river bending and winding... sections.

- Nothing there. Negative again.

- Hold it. I think we may have something. Let's take a closer look.

- That may be it.

[...]